Analysis Note

ControlRecommended positive control tissue: Neuroblastoma

Application

Research Sub CategoryGrowth Cones & Axon GuidanceNeurofilament & Neuron Metabolism

Research CategoryNeuroscience

Immunohistology on frozen tissue sections acetone fixation only.

Immunoblotting: Recognizes 120, 140, and 180 kDa NCAM isoforms.

ELISA: 80 ng/mL

Does not work in flow cytometry.

Optimal working dilutions must be determined by end user.

Anti-NCAM Antibody, extracellular, clone ERIC-1, Azide free is an antibody against NCAM for use in ELISA, WB, IH.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Human Retinoblastoma tumor

Epitope: extracellular

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MAB2122

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Liquid in PBS. Does not contain any preservatives

Format: Purified

Specificity

Recognizes N-CAM expressed on developing and adult neuroectrodermal tissues in Humans. Neuroectodermal tumors also stain including Elioma, ependymoma, neuroblastoma, medulloblastoma, retinoblastoma and teratoma. Oat cell carcinoma and Wilms tumor are also highly reactive. Will react on Natural Killer cells. Recognizes 120, 140 and 180 kDa NCAM isoforms.

Storage and Stability

Store at -20°C only. Avoid repeated freeze-thaw cycles.